Swietenia mahagoni seed oil and Method of extraction

Abstract:An aspect of the present invention to provide a method for extraction of seed oil of swietenia mahagoni comprising the step of; providing ground swietenia mahagoni seed, subjecting the ground swietenia mahagoni seed to a supercritical carbon dioxide exraction system, obtaining oil extract of swietenia mahagoni see

Extraction and characterisation of pectin from dragon fruit (hylocereus polyrhizus) peels

Pectins are complex carbohydrate molecules that are used in numerous food applications as a gelling agent, thickener, stabiliser, and emulsifier. Dragon fruit (Hylocereus polyrhizus) is one of the tropical fruits that belong to the cactus family, Cactaceae. Since the peels of dragon fruit are often discarded as waste, it would be an advantage to convert it into a value-added product such as pectin. The objective of this study was to investigate the extraction of pectin from dragon fruit peels under different extraction time using hot water extraction method. The dragon fruit peels were extracted using distilled water at 80 °C with different extraction time of 20, 40, 60 and 80 min. The extracted pectin was characterised by its yield, moisture and ash content, degree of esterification and antioxidant activity. Determination of moisture and ash content was conducted using AOAC standard method. The determination of the degree of esterification of pectin was performed using Fourier Transform Infrared Spectroscopy (FTIR). DPPH assay was used to determine the antioxidant activity of the pectin extract. Based on the result, the yield of pectin decreases (20.34 to 16.20 %) with the increase of extraction time, moisture contents were between 4 to 6 % while ash contents were between 7 to 10 %. Pectin from dragon fruit peels was determined as low methoxyl pectin and has high percentage of antioxidant activity with low value of inhibition concentration (IC50) (0.0063 to 0.0080 mg/mL). 60 min extraction sample exhibits the highest antioxidant activity (81.91 % at 40 μg/mL), followed by 80 min extraction (81.68 % at 40 μg/mL), 40 min extraction (81.38 % at 40 μg/mL) and 20 min extraction (81.31 % at 40 μg/mL)

THE EFFECT OF SUPERCRITICAL CO2 ON THE ANTIOXIDANT ACTIVITY OF THE SWIETENIA MAHAGONI SEED EXTRACT BY BOX-BEHNKEN DESIGN

In this study, the influence of supercritical carbon dioxide parameter on the antioxidant activity on Swietenia mahagoni seed extract was investigated. Response surface methodology was employed, using Box-Behnken statistical design to evaluate the effects of three independent variables. Pressure (20-30 MPa), temperature (40-60 oC) and particle size (0.25-0.75 mm) were the three variables with total time and CO2 flow rate being held constant at 180 minutes and 2 ml/min respectively, for each extraction. The extracts were screened for possible antioxidant activitiy by free radical scavenging activity 2,2-diphenyl-1-picryl hydrazyl (DPPH) assays. The study reveal that the highest antioxidant activity which was 94.84% was obtained at temperature of 40oC, pressure of 25 MPa and particle size of 0.25 mm. They stated that the dominan effect on the antioxidant activity of the Swietenia mahagoni seed extracts was the pressure campared to temperature and particle size

Jenis Pelarut Ekstraksi Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Aktivitas Antioksidan dan Antibakteri

Dalam penelitian ini kami mengevaluasi antioksidan dan aktivitas antibakteri biji Swietenia mahagoni dengan menggunakan berbagai jenis pelarut. Ekstrak disaring untuk menguji aktivitas antioksidan radikal bebas scavenging 2,2- difenil -1-Pikril hydrazyl (DPPH). Total fenol juga telah ditentukan. Ekstrak metanol dari biji Swietenia mahagoni menunjukkan aktivitas DPPH tertinggi (60.89 %), bila dibandingkan dengan ekstrak  metanol 70 % (60,77 %), etanol 100 % (58,85 %), etanol 70 % (59.87 %) dan aseton 100% (46.54 %). Jumlah kandungan fenol ditentukan dengan teknik spektrofotometri, menggunakan reagen Folin-Ciocalteau. Hasil penelitian menunjukkan bahwa ekstrak etanol 100% memiliki kandungan fenolik yang lebih tinggi (67 mg/g sampel ) dibandingkan dengan jenis pelarut lain (etanol 70 %: 65 mg/g sampel, metanol 100 %: 63 mg/g sampel, metanol 70 %: 55 mg/g sampel , aseton 100%: 54 mg/g sampel). Aktivitas antibakteri terhadap bakteri Gram-positif (Bacillus subtilis, Staphylococcus aureus) dan bakteri Gram-negatif (E. coli, Pseudomonas aeruginosa) diuji berdasarkan zona penghambatan dengan metode difusi disk. Streptomisin sebagai antibakteri standar (kontrol positif). Hasil menunjukkan bahwa biji Swietenia mahagoni dengan ekstraksi pelarut metanol, etanol dan aseton memiliki efek penghambatan terhadap pertumbuhan Bacillus subtilis, Staphylococcus aureus, E. coli dan Pseudomonas aeruginosa pada konsentrasi 100 mg/ml

Response Surfaces of Linoleic Acid of Swietenia Mahagoni in Supercritical Carbon Dioxide

Abstract. The process variables pressure, temperature and particle size were studied for optimization of linoleic acid by response surface methodology following a Box-Behnken design of experiments. The results indicated the effect of extraction condition on linoleic acid of the extracts produced SC-CO2 gave the different effect. But, Analysis of the variance of the data indicated that there was no statistically significant difference between the other sample. Although there was greater variation within the sample, there was still no statistically significant effect of temperature and pressure on the extraction. The optimum conditions for linoleic acid yield from Swieteniamahagoni seed within the experimental range were found to be pressure of 29.02 MPa, a temperature of 67.88ºC and particle size 0.75 mm, and the predicted linoleic acid was found to be 34.91%

Evaluation of Swietenia mahagoni Jacq seed extracts in promoting wound healing properties

Swietenia mahagoni or known as tunjuk langit is a widely known plant to possess good properties in treating diseases as well as a wound treatment. The purpose of this work was to examine the wound healing ability of the seed extracts in term of its ability to promote cell proliferation and migration. The extracts from two extraction methods, i.e. supercritical carbon dioxide and Soxhlet, were evaluate using cytotoxicity and scratch assays on human skin fibroblast cells. The findings showed that the extraction yield using supercritical fluid extraction was lower than Soxhlet method with 48.9% yield recovery. In addition, the seed extracts were able to stimulate cell growth and migratory effect. This information can be used as a basis to performed subsequent study to report wound healing activity of this plant material

Supercritical fluid extraction of bioactive flavonoid from Strobilanthes crispus (pecah kaca) and its comparison with solvent extraction

Supercritical carbon dioxide extraction (SC-CO 2) of bioactive flavonoid from Strobilanthes crispus (Pecah Kaca) was performed to study the effects of various parameters such as pressure, temperature and dynamic extraction time on the yield and composition of bioactive flavonoid. The results were also compared with those obtained by conventional Soxhlet extraction in lab conditions. The results from SFE showed that the effect of extraction variables on extraction yields decreased in the following order: pressure, temperature and dynamic extraction time. The extraction pressure played a dominant role in the yield of the sample while the effect of time could be ignored. This study also revealed that both Soxhlet extraction and SC-CO 2 extraction can be used to obtain flavonoid compound. Under the optimum conditions, the highest bioactive flavonoid compound content was obtained at 3.98% and eight flavonoid compounds were identified by HPLC

Perah Oil: A Potential Substitute for Omega-3 Oils and its Chemical Properties

Abstract: The perah seed oil is a potential source for high unsaturated and omega 3 oil. It can be an alternative choice for regular vegetable oil. The chemical and physical properties need to be investigated and evaluated for it to be used feasibly in the food industries and health care products. Modern day's faster and sophisticated instruments and techniques are used to evaluate the physicochemical properties of edible or vegetable oils. This study includes FTIR spectroscopy GC-MS and GC to evaluate the chemical properties of it while colour was used to study the physical property. The peaks by FTIR were found at 3300-3000 cm-1, 2960-2850 cm-1, 1760-1670 cm-1, 1650-1580 cm-1, and 1340-1020 cm-1. The GC-MS discover the potential beneficial compounds that available in extracted perah oil which are squalene, Vitamin E,and L.beta.-sitosterol. The GC data demonstrate about the free fatty acid profile which showed the high unsaturation in all oil samples extracted by different solvents. However, the colour measure for the visual suitable acceptability use for food or cosmetic application

WOUND HEALING PROPERTIES OF SWIETENIA MAHAGONI SEED EXTRACTED USING SCCO2: AN IN VITRO STUDY

Abstract: Swietenia mahagoni seed has been traditionally used as a wound healing agent. The aim of this study was to investigate the in vitro healing properties of Swietenia mahagoni seed extract. A supercritical carbon dioxide (SCCO2) extract of Swietenia mahagoni seeds was tested regarding its ability to stimulate the growth of human skin fibroblasts (HSF 1184) in vitro using the colorimetric methylthiazol tetrazolium (MTT) assay and scratch assay in a selected range of extract concentrations. The results show that the Swietenia mahagoni seed extract at a concentration of 0.01 mg/mL was able to stimulate the growth of human skin fibroblasts. The scratch assay showed that all of the extracts led to a significantly higher percentage of wound closure compared to the control. The wound healing capabilities of Swietenia mahagoni seed extract may be due to its fibroblast stimulation activity, which may promote repair of the wounded dermis. Thus, it may serve as a good herbal component to incorporate into products designed to promote wound healing

PENGARUH JENIS PELARUT EKSTRAKSI BIJI MAHONI (Swietenia mahagoni Jacq) TERHADAP AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI

Abstrak: Dalam penelitian ini kami mengevaluasi antioksidan dan aktivitas antibakteri biji Swietenia mahagoni dengan menggunakan berbagai jenis pelarut. Ekstrak disaring untuk menguji aktivitas antioksidan radikal bebas scavenging 2,2- difenil -1-Pikril hydrazyl (DPPH). Total fenol juga telah ditentukan. Ekstrak metanol dari biji Swietenia mahagoni menunjukkan aktivitas DPPH tertinggi (60.89 %), bila dibandingkan dengan ekstrak metanol 70 % (60,77 %), etanol 100 % (58,85 %), etanol 70 % (59.87 %) dan aseton 100% (46.54 %). Jumlah kandungan fenol ditentukan dengan teknik spektrofotometri, menggunakan reagen Folin-Ciocalteau. Hasil penelitian menunjukkan bahwa ekstrak etanol 100% memiliki kandungan fenolik yang lebih tinggi (67 mg/g sampel ) dibandingkan dengan jenis pelarut lain (etanol 70 %: 65 mg/g sampel, metanol 100 %: 63 mg/g sampel, metanol 70 %: 55 mg/g sampel , aseton 100%: 54 mg/g sampel). Aktivitas antibakteri terhadap bakteri Gram-positif (Bacillus subtilis, Staphylococcus aureus) dan bakteri Gram-negatif (E. coli, Pseudomonas aeruginosa) diuji berdasarkan zona penghambatan dengan metode difusi disk. Streptomisin sebagai antibakteri standar (kontrol positif). Hasil menunjukkan bahwa biji Swietenia mahagoni dengan ekstraksi pelarut metanol, etanol dan aseton memiliki efek penghambatan terhadap pertumbuhan Bacillus subtilis, Staphylococcus aureus, E. coli dan Pseudomonas aeruginosa pada konsentrasi 100mg/ml